Allelophatic and Antioxidant Activity of Eight Amazon Species from De Tamshiyacu Tahuayo Reserve.
Abstract
In this study were evaluated the antioxidant activity (AAO), allelopathic (ALL) and bioactive substances present in leaves of eight plant species from the Tamshiyacu-Tahuayo Community (Loreto-Peru). The AAO evaluation was performed by the free radical DPPH capture method, using dry and pulverized leaves, macerated in methanol for fifteen days, solutions of 0.01- 5.0 mg/mL and 0.1 mM DPPH were prepared, reading was performed in the UV/vis spectrometer at 517 nm, for the ALL was performed by the Petri plates method, using methanolics extracts of fresh leaves with concentrations of 0.1- 50.0 mg/mL against the growth of pregerminated seeds of Latuca sativa , the reading was made by measuring the hypocotyl and radicle of the seedling. In the results of species with high AAO were Virola sebifera, Caryocar glabrum and Tapirira guianensis, which present a percentage of inhibition superior to 50% in concentrations of 5.0 mg/mL. The species with high ALL are Xylopia benthami, Malouetia naias and Virola surinamensis, reaching an EC50 for the radicle at concentrations lower than 1.0 - 50.0 mg/L. The concentration of phenolic compounds for these species varied from 115217,18-321815,14 mg/kg, flavonoids from 1030,79-1441,16 mg/kg and proanthocyanidins from 0.65-1,77 mg / 100g. Total alkaloids range from 0,0 - 68,2 m/g evaluation, presenting differences between them.
Full Text: PDF DOI: 10.15640/jns.v7n1a4
Abstract
In this study were evaluated the antioxidant activity (AAO), allelopathic (ALL) and bioactive substances present in leaves of eight plant species from the Tamshiyacu-Tahuayo Community (Loreto-Peru). The AAO evaluation was performed by the free radical DPPH capture method, using dry and pulverized leaves, macerated in methanol for fifteen days, solutions of 0.01- 5.0 mg/mL and 0.1 mM DPPH were prepared, reading was performed in the UV/vis spectrometer at 517 nm, for the ALL was performed by the Petri plates method, using methanolics extracts of fresh leaves with concentrations of 0.1- 50.0 mg/mL against the growth of pregerminated seeds of Latuca sativa , the reading was made by measuring the hypocotyl and radicle of the seedling. In the results of species with high AAO were Virola sebifera, Caryocar glabrum and Tapirira guianensis, which present a percentage of inhibition superior to 50% in concentrations of 5.0 mg/mL. The species with high ALL are Xylopia benthami, Malouetia naias and Virola surinamensis, reaching an EC50 for the radicle at concentrations lower than 1.0 - 50.0 mg/L. The concentration of phenolic compounds for these species varied from 115217,18-321815,14 mg/kg, flavonoids from 1030,79-1441,16 mg/kg and proanthocyanidins from 0.65-1,77 mg / 100g. Total alkaloids range from 0,0 - 68,2 m/g evaluation, presenting differences between them.
Full Text: PDF DOI: 10.15640/jns.v7n1a4
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